Evaluation and comparison of four protein extraction protocols for mono- and two-dimensional electrophoresis in Mytilus galloprovincialis
In this study, four protein extraction protocols from Mytilus galloprovincialis were evaluated with the aim to identify the most practical, efficient and Presser Feet reproducible method.Four extraction protocols frequently used for mussels and organic matrices were selected and compared.The methods were based on the use of: i) TRIzol reagent; ii)